WebMay 20, 2014 · The Mg-protoporphyrin IX methyltransferase (CHLM) gene encodes a subsequent enzyme in the chlorophyll synthesis pathway. We examined stomatal movement in a CHLM knockdown mutant, chlm, and found that it also exhibited an ABA-insensitive phenotype. However, lost1 and chlm seedlings all showed normal expression of ABA … WebA bacteriochlorophyll a biosynthesis mutant of the purple photosynthetic bacterium Rhodobacter capsulatus was functionally complemented with a cosmid genomic library from Synechocystis sp. PCC 6803. The complemented R. capsulatus strain contains a defined mutation in the bchM gene that codes for Mg-protoporphyrin IX methyltransferase, the …
S-adenosyl-L-methionine:magnesium-protoporphyrin IX O-methyltransferase …
WebTIGR02024 (PSSM ID: 273930): Conserved Protein Domain Family BchM-ChlM, This model represents the S-adenosylmethionine-dependent O-methyltransferase responsible for methylation of magnesium protoporphyrin IX WebResults: The SAM- and SAH-bound ChlM structures were obtained, and the ChlM active site was characterized. Conclusion: Flexibility of two regions could be a key modulator for methyltransferase activity. Significance: This work provides first structural insights into the catalytic mechanism of ChlM. Magnesium protoporphyrin IX O ... cheap infant jordan clothes
KEGG ORTHOLOGY: K03428 - Genome
WebJul 30, 2014 · Magnesium protoporphyrin IX O-methyltransferase (ChlM) catalyzes transfer of the methyl group from S-adenosylmethionine to the carboxyl group of the C13 propionate side chain of magnesium protoporphyrin IX.This reaction is the second committed step in chlorophyll biosynthesis from protoporphyrin IX. Here we report the … WebInteractions between the methyltransferase and mag-nesium chelatase are likely to be crucial in determining the availability of MgP for both signalling [20] and biosynthetic roles in the chloroplast. Results ChlH stimulates the methyltransferase reaction ChlM (0.2 lm) was assayed in the presence of 50 lm MgD, 1 mm SAM and varying concentrations of WebSep 15, 2007 · The ChlM protein from Synechocystis PCC 6803 complemented a BchM mutant of R. capsulatus . Most recently Synechocystis PCC 6803 ChlM has been heterologously expressed in E. coli and purified to homogeneity . The reaction mechanism of methyltransferase appears to vary between a plant, algal and photosynthetic bacterium. cheap infant halloween costumes