Web1.2 ug of DNA was digested with DNase I for 12 minutes. The digested DNA was tagged using that BioPrime Array CGH Genomic Labeling Element with Amersham cUTP-CY dyes upon GE Healthcare (Reference=CY3 and Tumor=CY5). The labeled DNA were purified using Vivaspin 500 columns : Channel 2: Sourced name: Promega XX Control DNA: WebFrozen testis tissues were homogenized, and total RNA extracted using the miRNeasy Micro Kit (Qiagen, Germany), according to the manufacturer’s protocol. ... 1 μL of each primer and 7.0 μL of DNase/RNase-free water for the gene expression profile. β-actin was used as …

ATAC-Seq-based Identification of Extrachromosomal Circular …

WebDNases, or deoxyribonucleases, are enzymes that specifically cleave and degrade DNA. In molecular biology, DNase (namely DNase I) is used to degrade DNA in applications such as RNA isolation, reverse transcription preparation, DNA-protein interactions, cell culture, … WebThe leftover eccDNA was then cleaned with MinElute Reaction Cleanup Kit (Qiagen) after linear DNA was removed. A 20 μL of pure circular DNA is ultimately yielded, ... The proportion of eccDNA intersected with DNase clusters, H3K27Ac, H3K4Me3, H3K4Me1, … nrs 108 mechanic\u0027s lien release

RNase-Free DNase Set - 1500units for 50 RNA minipreps - RSC …

WebRNase-Free DNase Set Product Sheet. Share. For DNase treatment with QIAGEN or PreAnalytiX RNA purification kits. pdf. 35KB. English. Format. File size. Language. WebApr 19, 2016 · Set up the DNase digestion reaction as follows: RNA in water or TE buffer 1–8µl RQ1 RNase-Free DNase 10X Reaction Buffer 1µl RQ1 RNase-Free DNase 1u/µg RNA Nuclease-free water to a final ... Web1. Dilute DNase I 10X Reaction Buffer to 1X using RNase-Free water. 2. Prepare 50 µL of a working DNase I Solution for each sample to be treated by adding 5 µL of RNase-Free DNase I to 45 µL of 1X Reaction Buffer (from Step 1). 3. Completely re-suspend 5 μg of a … nrr with double hearing protection