Klf2 shrna

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August undefined, 2024

WebApr 25, 2024 · Silencing KLF2 with KLF2-specific shRNA aggravates the damage to hyperoxia-caused cell viability. In addition, as illustrated in Figures 6(d)–6(g), KLF2 shRNA … WebMethods: Genetic manipulations of KLF2 were performed in bEnd.3 cells and SCI model. Western blot, qRT-PCR, immunofluorescence staining and Lyso-Tracker Red staining, Evans blue dye extravasation, behavioral assessment via Basso mouse scale (BMS), electrophysiology and footprint analysis were performed.

The pharmaceutical solvent N-methyl-2-pyrollidone (NMP …

WebHere, we demonstrate a novel mechanistic relationship between p66shc and the kruppel like factor-2 (KLF2) transcription factor and show that this relationship has biological … WebMay 7, 2024 · In select experiments, HCAEC were first transduced for 24 h with a rAd expressing a human KLF2 silencing short hairpin (Ad-h-KLF2-shRNA) or a control rAd comprising a scramble short shRNA sequence (Ad-GFP-U6-shRNA) at a MOI of 100 (Vector Biolabs, Malvern, PA). greengate caravan park porthmadog

Original Article Methylation of kruppel-like factor 2 (KLF2) …

WebMay 3, 2010 · Klf2-expressing retrovirus and shRNA-expressing lentivirus. A mouse Klf2-expressing retroviral vector was prepared by PCR-based cloning of mouse Klf2 cDNA into a mouse stem cell virus–based vector and IRES2-EGFP that are expressed under the viral endogenous promoter. The virus was assembled in Phoenix cells provided by Garry Nolan … WebAug 27, 2015 · As expected, single knock down of either gene has no obvious effect on mESC self-renewal in 2i condition (data not shown). We then selected the shRNAs with the highest knockdown efficiency (Klf2 … WebMar 2, 2015 · The patients were divided into a low KLF2 expression group (n=59) and a high KLF2 expression group (n=21) according to whether their samples showed up or downregulation of TINCR compared with the ... greengate business park valley road

(PDF) Endothelial Klf2-Foxp1-TGFβ signal mediates the inhibitory ...

Silencing of Kruppel-like factor 2 by the histone ... - Nature

WebSep 5, 2011 · Conversely, stable depletion of KLF2 by shRNA in the non-transformed MRC5 fibroblast cells increased cell viability as assessed by the MTT assay . We also wished to investigate the contribution of ... green gate caister on seaWebMar 3, 2024 · MAP3K2 is a serine/threonine kinase belonging to the MAP3K superfamily 11. To investigate the role of MAP3K2 in the intestine, we added dextran sodium sulfate (DSS), which causes colitis, to the... greengate calgary

"WebSep 1, 2024 · For shRNA-mediated knockdown of Klf2 and Snd1, DNA fragments targeting Klf2 and Snd1 were subcloned into pLKO.1-TRC vector (Addgene). Lentiviral vectors were co-transfected into HEK293T cells together with pCMV-VSV-G (Addgene) and psPAX2 (Addgene) plasmids using Lipofectamine 3000 reagent (Invitrogen), and viruses were … " - Klf2 shrna

Klf2 shrna

WebNov 23, 2024 · Background: Circular RNAs (circRNAs), as a class of endogenous RNAs, are implicated in osteosarcoma (OS) progression. However, the functional properties of circDOCK1 in OS have been largely unexplored. The present study demonstrated the regulatory mechanism of circDOCK1 in OS. Methods: QRT-PCR and Western blots were … WebHuman KLF2 (10365), Mouse Klf2 (16598), Rat Klf2 (306330), chicken... Summary: Kruppel-like factors (KLFs) are a family of broadly expressed zinc... Related Products: Antibodies …

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Web专利汇可以提供用于穿过维管结构的基因转移的方法和组合物专利检索，专利查询，专利分析的服务。并且本公开提供了在氨基酸序列中包含修饰的aav衣壳蛋白，以及包含经修饰的aav衣壳蛋白的病毒衣壳和病毒载体。 WebAug 30, 2024 · The binding of EZH2 to KLF2 promoter was verified by chromatin immunoprecipitation assay. ... (NC) for short hairpin RNA (shRNA) (the sh-NC group), while the remaining 8 mice were treated with ...

WebSep 8, 2015 · Klf2 and Tfcp2l1 Knock Down Impairs 2i-Mediated mESC Self-Renewal (A) qRT-PCR analysis of Klf2 expression in Klf2 shRNA knockdown 46C mESCs. Data represent mean ± SD of three biological replicates. ∗∗ p < 0.01 versus scramble shRNA. (B) Western blot analysis of KLF2 expression in Klf2 shRNA knockdown 46C mESCs. WebSilencing of KLF2 with KLF2 shRNA abolished the effects of laquinimod in inhibiting the expression of endothelial adhesion molecules and attachment of U937 monocytes to …

Webrally delivered KLF2 shRNA inhibited KLF2 mRNA by 50% to 60% compared to an empty lentiviral vector (Figure 1D) or a nonsilencing siRNA control vector (supplemental Figure II). Although KLF2 knockdown under shear did not affect Nrf2 mRNA, it reduced expression levels of NQO1 (P 0.01) and showed a decreasing trend in HO-1 expression compared WebApr 11, 2024 · The shRNA for human CUX1 based on the previous study [28] was packaged into lentivirus by GenePharma Co., Ltd. (Shanghai, China). Cells were transfected with lentivirus carrying shRNA-CUX1 or negative control sequences for 24 h and then cultured in a new DMEM medium for 48 h. ... Klf2, Klf4, and Klf6 were found to be the key TFs in …

WebMar 21, 2024 · KLF2 (KLF Transcription Factor 2) is a Protein Coding gene. Diseases associated with KLF2 include Cerebral Cavernous Malformations and Spleen Cancer . …

WebFeb 10, 2008 · Normal undifferentiated ES colonies with positive alkaline phosphatase staining were maintained after knockdown using two shRNA constructs targeting different regions of Klf2 or Klf5, respectively. flushots 2023 manitobaWebApr 15, 2024 · However, SK-MES-1 cells transfected with KLF2 shRNA#3 or shRNA#4 had a significant decrease in the G0/G1 phase but the cell cycle progressed into the S and G2/M phase (Figure 5I). To further explore the underlying mechanisms of KLF2 re-expression involved in cell cycle arrest at the G0/G1 phase we performed western bolt analysis of p15 … greengate care homeWebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. green gate cafe cheraw scWebApr 15, 2024 · Western blot was performed to analyze the effects of KLF2 shRNA on knockdown of KLF2 expression and p15 and p21 expression in cells. We found that KLF2 … greengate butchersWebactivity of KLF2 and ATF2 transcription factors, resulting in quiescent and healthy endothelium.3–7 However, as other molecular factors may also play a role, we recently established the speciﬁc gene expression in endothelium overlaying human atherosclerotic lesions, to identify genes with a potential to modulate intimal accumulation of ... flu shot researchWebFAM83A and DDIT4 expression was knocked-downed in leukemia cells via lentivirus-shRNA. Western blotting, RT-qPCR, cell cycle analysis and apoptosis assays were used to determine the effect of lovastatin-induced growth suppression in leukemic cells in vitro. ... We further show that KLF2 induction by lovastatin is responsible for lower ... flu shots 2022-23 visWebMay 8, 2008 · KLF2 induces the inhibitory SMAD7 and reduces nuclear levels of the AP-1 member c-Jun, thereby fully suppressing TGF-β–driven gene expression. 6 A third cluster showed enrichment for the antioxidant response element (ARE), suggesting a role for nuclear factor erythroid 2-like 2 (Nrf2) that binds the ARE. 3 green gate cafe glasgow