Klf4 chip-seq

Author: gwwf

August undefined, 2024

WebAnti-KLF4, Cat. No. ABE2860, is a highly specific rabbit polyclonal antibody that targets Krueppel-like factor 4 (Klf4) and has been tested for use in ChIP-seq, Chromatin … WebDec 14, 2024 · While maintaining KLF4 function as a reprogramming factor, L507A variant additionally showed distinct DNA-binding patterns and downstream gene regulation from WT. Especially, we identified that KLF5 …

KLF4 recruits SWI/SNF to increase chromatin accessibility and reprogra…

WebDec 11, 2024 · ChIP-seq data and analysis. For ChIP-seq analyses, 1x10 7 cells of the Akt transformed prostate stem cell line with endogenous Klf4 were fixed in PBS containing 1% of formaldehyde for 8 min at room temperature on a rotating platform. The reaction was quenched with 0.125M glycine for 5 min. WebFeb 3, 2015 · A genome-wide RNA-seq analysis was conducted to identify genes regulated by KLF4 in T-ALL cells. Chromatin immunoprecipitation (ChIP) PCR was used to determine direct binding sites of KLF4 in T-ALL cells. Here we reveal that KLF4 induced apoptosis through the BCL2/BCLXL pathway in human T-ALL cell lines and primary T-ALL specimens. pa tax form 1000

KLF4 Recruits SWI/SNF to Increase Chromatin Accessibility …

WebWe reaffirm the results obtained from in silico and chromatin studies in SCC25 by ChIP-seq of KLF4 and identify ΔNp63 as a co-oncogenic driver of the cancer-specific gene … WebDec 7, 2024 · with KLF4-ChIP seq data, we observed KLF4 binding en-richment at transcription start sites (TSSs) of more than. 50% of genes that were signi cantl y changed in NPCs com-pared with ESCs (fold ... WebJul 17, 2024 · Remarkably, we provide evidence that Klf4 and Oct4 control nearly opposite patterns of gene expression in SMC and based on in vivo ChIP-seq analyses have … tiny house sewage options

KLF4-dependent phenotypic modulation of smooth muscle cells ... - PubMed

Klf4 Is a Transcriptional Regulator of Genes Critical for EMT, Including …

WebOn the basis of in vivo KLF4 chromatin immunoprecipitation-sequencing (ChIP-seq) analyses and studies of cholesterol-treated cultured SMCs, we identified >800 KLF4 target … WebDec 22, 2009 · The recent development of ultra-high-throughput RNA sequencing (RNA-Seq) technology holds the promise to provide more accurate gene expression measurements (1, 2).Compared with microarray, RNA-Seq is able to measure absolute concentration of transcripts ().Meanwhile, chromatin immunoprecipitation (ChIP) coupled with microarray … pa taxes on social securityWebOct 5, 2024 · ChIP-seq and CLIP-seq) is crucial in identifying transcription factor (TF) binding sites and inferring gene regulatory mechanisms for any organism. Since 2015, deep learning (DL) methods have been widely applied to identify TF binding sites and predict motif patterns, with the strengths of offering a scalable, flexible and unified computational ... tiny houses florence sc

"WebOn the basis of in vivo KLF4 chromatin immunoprecipitation-sequencing (ChIP-seq) analyses and studies of cholesterol-treated cultured SMCs, we identified >800 KLF4 target genes, including many that regulate pro-inflammatory responses of SMCs. " - Klf4 chip-seq

Klf4 chip-seq

KLF4 is involved in the organization and regulation of

Web2 days ago · RNAPII ChIP-seq from GSE98605 . n = 3 merged replicates per condition, shown as a single heatmap. 43,152/45,518 RNAPII ChIP-seq peaks directly overlapped a called “consensus” OLDN peak (94.8%). Gel-extracted libraries were used for these analyses and size-selected for fragments between 230–270 bp. Data were analyzed for … WebJan 19, 2024 · Cooperative binding of Oct4, Sox2, and Klf4 with stage-specific transcription factors orchestrates reprogramming [ChIP-seq] Oct4, Sox2, Klf4, and cMyc (OSKM) …

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WebJul 3, 2013 · To further explore the molecular mechanisms of Klf4’s role in mediating long-range interactions, we focused on the potential interacting partners of Klf4 in PSCs. Previous ChIP-seq data have demonstrated that Klf4 colocalizes with cohesin complex (Nitzsche et al., 2011), which has been shown to be a critical player in DNA looping (Kagey et al ... WebJul 10, 2024 · KLF4 ChIP-Seq. We demonstrated, using KLF gain-and-loss-of-function experiments, that KLF is required to mediate chromatin accessibility changes at proximal and distal sites, adding to the known role for KLF in transcriptional activation of flow responsive genes.

WebDec 7, 2024 · KLF4 binds to neural differentiation-associated gene loci that carry poised enhancer marks. To determine whether KLF4 regulates interactions between gene regulatory elements and target genes, we first analyzed RNA-seq data in mESCs and mouse neural progenitor cells (NPCs) (41). WebMar 7, 2013 · The ChIP-seq data at the Klf4 gene locus revealed a distal region with multiple IRF8 ChIP-seq peaks spanning 120 kb (208 to 328 kb upstream of the Klf4 TSS) ( Figure 3A and supplemental Figure 2). Each IRF8 ChIP-seq …

WebSep 3, 2024 · Using mice with podocyte-specific knockdown of Klf4, we conducted glomerular RNA-sequencing, tandem mass spectrometry, and single-nucleus RNA-sequencing to identify cell-specific transcriptional changes that … Web( c) Heat maps of KLF4, ZNF750, and CTCF ChIP–seq signal on day 3 at enhancers with gained H3K27ac signal. ( d) Box-and-whisker plots representing relative H3K27ac ChIP–seq signal in control versus transcription factor–knockdown conditions, for two biological replicates of each knockdown.

WebKruppel-like factor 4 (KLF4; gut-enriched Krüppel-like factor or GKLF) is a member of the KLF family of zinc finger transcription factors, which belongs to the relatively large family of …

WebFeb 25, 2013 · Klf4 Is a Transcriptional Regulator of Genes Critical for EMT, Including Jnk1 (Mapk8) We have identified the zinc-finger transcription factor Kruppel-like factor 4 (Klf4) … pa tax file websiteWebFeb 25, 2013 · A combinatorial approach of gene expression profiling and chromatin immunoprecipitation/deep sequencing (ChIP-Seq) analysis revealed many key EMT … pa tax extension 2021 onlineWebJun 5, 2014 · Analysis of Tcf3 genomic binding sites through chromatin immunoprecipitation and high-throughput sequencing (ChIP-seq) reveals that Gsk3 signaling inhibition alleviates Tcf3-mediated transcriptional repression of … pa tax file online freeWebMay 10, 2024 · Remarkably, the analysis of KLF4-ChIP-seq data revealed a much larger fraction of the promoter and enhancer regions of Agos_sDEGs that were bound by KLF4 … pa tax form schedule 1WebApr 11, 2024 · Furthermore, the authors identified the Dlx3, Klf4, ... ChiP-seq analyses of the Dlx3 genome-wide binding showed predominance of the Dlx3 peaks in intergenic regions (47%) over the proximal promoters (15%), suggesting a role for Dlx3 in long-range chromatin interactions. Importantly, further correlation of the peaks enriched for both Dlx3 and ... pa taxes on 401k withdrawalWebMay 12, 2011 · ChIP-seq analyses of RAD21 reveal an ESC specific cohesin binding pattern that is characterized by CTCF independent co-localization of cohesin with pluripotency related transcription factors Oct4, Nanog, Sox2, Esrrb and Klf4. pa tax form schedule dWebKLF4 chromatin regulator Somatic tissue differentiation commonly requires the coordinated and simultaneous repression of progenitor genes mediating the proliferative cell program in concert with the activation of tissue-specific differentiation genes. Epidermal tissue contains pa tax filing online free