WebmCherry fluorophore filter set with bandpass emission for IX83/73/53 and BX63/53/43 microscopes. Standard 25mm-diameter filter set. Excitation: 565-585nm, Dichromatic Mirror: 595nm, Emission: 600-690nm. Common fluorophores: mCherry. SKU: N2738000. Price: Log in OR Register to see price. Quantity: WebIF. Confocal immunofluorescent analysis of A549 cells transiently transfected with an mCherry construct, using mCherry (E5D8F) Rabbit mAb and Anti-rabbit IgG (H+L), F (ab')2 Fragment (Alexa Fluor ® 488 …
Frontiers Construction of an Integrated mCherry Red Fluorescent ...
WebTransfection efficiency determined with fluorescence-based bottom reading for GFP and mcherry. Ann-Cathrin Volz, BMG LABTECH GmbH, Ortenberg, Germany, 02/2024. Transfection efficiency of cells can be reliably determined for GFP and mcherry on a plate reader. Spiral averaging gives speed advantages, while matrix scans provide local … Web2 nov. 2016 · I have generated an mCherry fusion protein construct in pCDNA3.0. The construct expresses in HEK293T cells as verifed by mCherry fluorescence and immunoblotting against mCherry. university of york sick leave
mCherry Fluorophore Filter Set (Standard 25mm-Diameter)
Web17 mrt. 2024 · Our results identified mCherry as a reliable and quantitative reporter for the study of gene expression in ... were vortexed at maximum speed with 300 mg of glass beads (400–600 μm diameter) for 15 s. The cells were spread on selective TP agar plates after mixing with 0.1% molten agar at 42 °C. The plates were incubated ... WebIn the wavelength range of mCherry (562/603 nm) there is nearly no background signal in wild type and GFP expressing cell line (Fig. 4B). It was possible to detect a significant … Web7 dec. 2024 · Fluorescence measurements of mCherry and its shorter isoforms (V1, V2 and V3). The 112 genetic map of each constitutively expressed mCherry version is shown … receive customer visit