Scycf1

Author: cjvm

August undefined, 2024

Webb14 apr. 2024 · Do not Store this antibody in 4°C. tested applications: Immunofluorescence (IF), Immunolocalization (IL), Western blot (WB) background: The Plasma Membrane … Webb1 feb. 1998 · On the basis of the functional resemblance of plant vacuolar GS conjugate transport to that mediated by ScYCF1 and HmMRP1 and the 44.6% sequence identity (63.9% similarity) between ScYCF1 and …

AtMRP1 gene of Arabidopsis encodes a glutathione S-conjugate …

Webbvacuolar ScYCF1 is important for Cd sequestration in Chlamydomonas reinhardtii TINGLIANG WANG & MADELINE WU Department of Biology, The Hong Kong University of … WebbSaccharomyces cerevisiae yeast cadmium factor (ScYCF1), a vacuolar glutathione-heavy metal(loid) conjugate, enhanced cad-mium (Cd) and lead (Pb) resistance and … thinkific help center

A mitochondrial half-size ABC transporter is involved in cadmium ...

Webb13 feb. 2006 · In this review, we will give a detailed overview of the mutation analysis reported for selected ABC transporters of the ABCB and ABCC subfamilies, namely HsCFTR/ABCC7, HsSUR/ABCC8,9, HsMRP1/ABCC1, HsMRP2/ABCC2, ScYCF1 and P-glycoprotein (Pgp)/MDR1/ABCB1 and their effects on the function of each protein. WebbScYCF1 is an ABC trans-porter of Saccharomyces cerevisae that contributes to Cd(II) resistance by pumping glutathione-conjugated cadmium(II) into the vacuole (Li et al., 1997), and SpHMT1, a half-size ABC transporter from Schizosaccharomyces pombe , contributes to WebbWe developed transgenic rice plants expressing two different vacuolar As sequestration genes, ScYCF1 and OsABCC1, under the control of the RCc3 promoter in the root cortical … thinkific growth plan

An ATP-binding cassette transporter related to yeast vacuolar …

Webbcerevisiae (ScYCF1) in the cotton, ScYCF1 was cloned from Saccharomyces cerevisiae As2.375, with the full-length of 4548 bp. The bioinformatics analy-sis revealed that the … Webb28 jan. 2024 · By contrast, three phosphorylation sites were detected in ScYCF1 [17, 18] and 10 in HsABCC7, also known as CFTR . However, the absence of altered phenotypes by single amino acid mutation does not exclude the possibility that these residues are involved in phosphorylation-mediated regulation. thinkific hkptrcWebbThe complementation of the Scycf1 strain was found BSO-sensitive, suggesting that cadmium conjugated to glutathione is a substrate of SpHMT1. Overexpression of a … thinkific hidden course

"WebbIn Saccharomyces cerevisiae, ScYCF1 represents a vacuolar membrane protein involved in heavy metal and drug detoxiﬁ-cation. Disruption of the gene results in a yeast strain hyper-sensitive to cadmium [37]. ScYCF1 is an ATP-dependent pump able to transport organic glutathione-S-conjugates (GS-conjugates) into the vacuole [38], GS–metal ... " - Scycf1

Scycf1

WebbScYcf1. dari ragi dan . HmMrp1. dari manusia. Komposisi PCR untuk . MaMrp. sama dengan untuk aktin, hanya primernya yang diganti dengan . mrpF. dan . mrpR. Kondisi PCR untuk . MaMrp. adalah sama dengan untuk aktin. Pengklonan fragmen cDNA . MaMrp. Fragmen cDNA . MaMrp. diligasikan dengan pGEM-T Easy (Promega Inc.) dengan … Webb1 juni 1998 · Abstract While the concept of H + -coupling has dominated studies of energy-dependent organic solute transport in plants for over two decades, recent studies have demonstrated the existence of a group of organic solute transporters, belonging to the ATP-binding cassette (ABC) superfamily, that are directly energized by MgATP rather …

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WebbDownload scientific diagram Gene amplification PCR product. M: DNA Marker DL5000; 1-2: ScYCF1 gene. from publication: Cloning and Ectopic Expression of ScYCF 1 Gene … Webb1 juli 1997 · On the assumption that the complete ORF of the gene corresponding to this cDNA was similar in size to those of ScYCF1 and HmMRP1, it was estimated to be missing a minimum of 1.5 kb of 5′ coding sequence. Hence, to isolate clones containing this missing sequence, 500 bp of the 5′ terminus of the 3.5-kb cDNA was used to screen two ...

Webb1 okt. 2006 · An ATP-binding cassette transporter related to yeast vacuolar ScYCF1 is important for Cd sequestration in Chlamydomonas reinhardtii. 1 Europe PMC requires … Webb18 mars 2024 · Up to now, more and more Hg tolerance genes such as ScYCF1, MerC-H and AtABCC1,2 have been identified, and we might co-transform these genes or their homologs into poplar for high-efficiency phytoremediation in the future. Competing financial interests. The authors declare that they have no conflict of interest.

WebbScYCF1 is alkaline and positive charged, stable, and hydrophilic protein. The predictive secondary structure is mainly composed of α-helix areas, random coils and β-sheets. We … Webb22 juli 1997 · (iii) The functional and structural equivalence of the Saccharomyces cerevisiae (yeast) cadmium factor (ScYCF1) gene product, identified according to its …

Webb6 apr. 2024 · Fixer composition and buffer: 4% (w/v) paraformaldehyde (freshly prepared as 8% stock and 0.2 µm filtered) 0.01% (v/v) Triton-X100 in Phosphate Buffered Saline …

Webb20 juli 2006 · When the cDNA of CrMRP2 was cloned into the yeast expression vector pEGKT and transformed into the yeast mutant strain DTY168 lacking ScYCF1, it restored … thinkific growth plan defining enrollment thinkific help phone numberWebbPCR dengan menggunakan primer untuk ekson1-ekson2 dari gen aktin menghasilkan DNA yang berukuran sekitar 450 pb (Gambar 2) yang menunjukkan bahwa daerah yang diamplifikasi adalah thinkific head officeWebbScYCF1 is alkaline and positive charged, stable, and hydrophilic protein. The predictive secondary structure is mainly composed of α-helix areas, random coils and β-sheets. We … thinkific icm loginWebbScYCF1 is alkaline and positive charged, stable, and hydrophilic protein. The predictive secondary structure is mainly composed of α-helix areas, random coils and β-sheets. We … thinkific get fredWebbThe chlorophyll content of the leaf discs processed was measured and results showed that under three kinds of treatment, the chlorophyll content of leaf discs in transgenic plants was thinkific glassdoorWebbComputer searches detected the significant resemblance of CrMRP2 with HsMRP1, AtMRP3 and ScYCF1, in Homo sapiens, Arabidopsis thaliana and Saccharomyces cerevisiae, respectively. All are members of the multidrug resistance-associated protein (MRP)/cystic fibrosis transmembrane conductance regulator (CFTR) subfamily of ATP … thinkific hello rache